5.4.99.11 additional information - - 3467 5.4.99.11 additional information - the specific activity of the Protaminobacter rubrum immobilized pellets in calcium alginate at 30°C range from 1.6 to 4.0 g isomaltulose/g pellet/h, respectively with 70% and 65% sucrose solution, while in lower sucrose concentration have higher specific activities presumably due to substrate inhibition of the isomaltulose synthase in higher sucrose concentrations 702716 5.4.99.11 0.0072 - pH 7.0, 30°C, purified recombinant mutant F297A+P 727307 5.4.99.11 0.04 - recombinant mutant AZF3, pH 6.0, 35°C, substrate isomaltulose 746984 5.4.99.11 0.046 - pH 7.0, 30°C, purified recombinant mutant F297A 727307 5.4.99.11 0.06 - recombinant mutant AZF3, pH 6.0, 35°C, substrate turanose 746984 5.4.99.11 0.11 - recombinant enzyme, pH 6.0, 35°C, substrate sucrose 746984 5.4.99.11 0.14 - pH 7.0, 30°C, purified recombinant mutant R333K 727307 5.4.99.11 0.22 - recombinant mutant AZF3, pH 6.0, 35°C, substrate sucrose 746984 5.4.99.11 0.52 - recombinant enzyme, pH 6.0, 35°C, substrate kojibiose 746984 5.4.99.11 0.6 - recombinant enzyme, pH 6.0, 35°C, substrate isomaltotriose 746984 5.4.99.11 0.97 - recombinant enzyme, pH 6.0, 35°C, substrate methyl alpha-D-glucoside 746984 5.4.99.11 0.98 - recombinant enzyme, pH 6.0, 35°C, substrate isomaltose 746984 5.4.99.11 1.03 - recombinant mutant AZF3, pH 6.0, 35°C, substrate 4-nitrophenyl alpha-D-trehalose 746984 5.4.99.11 1.03 - recombinant mutant AZF3, pH 6.0, 35°C, substrate 4-nitrophenyl-alpha-D-trehalose 746984 5.4.99.11 1.1 - pH 7.0, 30°C, purified recombinant wild-type enzyme 727307 5.4.99.11 2 - crude extract, in 25 mM PBS (pH 5.8), at 30°C 714486 5.4.99.11 2.16 - crude enzyme, pH 6.3, 33°C 726847 5.4.99.11 2.33 - recombinant enzyme, pH 6.0, 35°C, substrate isomaltulose 746984 5.4.99.11 2.9 - recombinant enzyme, pH 6.0, 35°C, substrate 4-nitrophenyl alpha-D-trehalose 746984 5.4.99.11 6 - free recombinant enzyme, pH 6.0, 30°C 746691 5.4.99.11 13.98 - substrate sucrose, pH not specified in the publication, 20°C, native enzyme 746878 5.4.99.11 19.8 - pH not specified in the publication, 40°C, native enzyme 746878 5.4.99.11 38.75 - purified native enzyme, pH 6.3, 33°C 726847 5.4.99.11 65.3 - sucrose 3467 5.4.99.11 71.58 - immobilized recombinant enzyme, pH 6.0, 30°C 746691 5.4.99.11 120 - native enzyme, pH not specified in the publication, 30°C 746878 5.4.99.11 181 - sucrose 3469 5.4.99.11 210 - - 690512 5.4.99.11 280 - sucrose 3466 5.4.99.11 328 - recombinant enzyme, pH not specified in the publication, 35°C 746878 5.4.99.11 351 - pH 6.0, 30°C 660758 5.4.99.11 351 - recombinant enzyme, pH not specified in the publication, 35°C 746878 5.4.99.11 423 - pH not specified in the publication, 30°C, native enzyme 746878 5.4.99.11 427.1 - after 213.6fold purification, in 25 mM PBS (pH 5.8), at 30°C 714486 5.4.99.11 500 - pH 5.5, 25°C 677398 5.4.99.11 562 - pH 6.0, 30°C 660758 5.4.99.11 562 - recombinant enzyme, pH not specified in the publication, 35°C 746878 5.4.99.11 900 - pH 5.5, 25°C 660622 5.4.99.11 900 - substrate sucrose, pH and temperature not specified in the publication, recombinant enzyme 746878 5.4.99.11 957.5 - purified recombinant wild-type enzyme, pH 6.0, 30°C, substrate sucrose 749077 5.4.99.11 1017.6 - purified recombinant mutant E175N pH 6.0, 30°C, substrate sucrose 749077 5.4.99.11 1045.7 - purified recombinant mutant K576D, pH 6.0, 30°C, substrate sucrose 749077 5.4.99.11 1218.9 - purified recombinant mutant E175N/K576D, pH 6.0, 30°C, substrate sucrose 749077 5.4.99.11 2362 - pH 6.0, 30°C 679557 5.4.99.11 2362 - recombinant enzyme, pH not specified in the publication, 35°C 746878