3.1.3.21 additional information - changes in glucose, glycerol and 2,3-butanediol determined in engineered bacteria, new metabolic pathway of glucose via glycerol to 1,3-propanediol constructed, effect of pH on production of 1,3-propanediol, effect of dihydroxyacetone and pH, comparison of enzyme activities growing on different substrates, successful introduction of a glycerol pathway of yeast into Klebsiella pneumoniae 687860 3.1.3.21 additional information - L-glycerol 3-phosphate (L-G3P) production via microbial fermentation, elimination of both GPP1 and GPP2 isoenzymes required, small-scale and large-scale batch fermentation, intracellular accumulation of L-glycerol 3-phosphate reaches high levels of about 200 mM, thereafter accumulation declines, extracellular L-glycerol 3-phosphate concentration increases throughout fermentation, most of the total L-glycerol 3-phosphate outside the cells, specific elimination of enzyme activity reveals considerable glycerol formation, unspecific dephosphorylation as a mechanism to relieve cells of intracellular L-glycerol 3-phosphate accumulation, final product titer of about 325 mg total L-glycerol 3-phosphate per litre of fermentation broth 685707 3.1.3.21 0.35 0.75 dependency on subcellular fraction 81027 3.1.3.21 0.63 - - 81031 3.1.3.21 0.8 - - 81033 3.1.3.21 6.1 - - 81030 3.1.3.21 46 - enzyme Gpp2p 81025 3.1.3.21 49 - enzyme Gpp1p 81025