2.8.1.1 additional information - - 662068 2.8.1.1 additional information - detection method for cyanide based on the enzymic reaction. Method shows a linear detection range from 0.015 to 0.5 mM with a detection limit of 0.003 mM and a limit of quantitation of 0.09 mM 675044 2.8.1.1 additional information - up to the 42nd day after a common bile duct ligation rhodanese of cytosol, mitochondria and microsom show a significant decrease in activity, activity of rhodanese in the serum increases markedly with a peak after one day and than decreases gradually to the control level 645540 2.8.1.1 0.003 - intestine 645546 2.8.1.1 0.008 - abomasum 645546 2.8.1.1 0.01 - abomasum and intestine 645546 2.8.1.1 0.01 - enzyme from lung, pH 9.2, 37°C 673175 2.8.1.1 0.01 - enzyme from muscular layer of rumen, pH 9.2, 37°C 673175 2.8.1.1 0.01 - enzyme from stomach, pH 9.2, 37°C 673175 2.8.1.1 0.012 - enzyme from muscle, pH 9.2, 37°C 673175 2.8.1.1 0.014 - intestine 645546 2.8.1.1 0.015 - with H2S as substrate 645535 2.8.1.1 0.016 - omasum 645546 2.8.1.1 0.017 - spleen 645546 2.8.1.1 0.018 - rumen 645546 2.8.1.1 0.02 - enzyme from lung, pH 9.2, 37°C 673175 2.8.1.1 0.02 - enzyme from muscle, pH 9.2, 37°C 673175 2.8.1.1 0.02 - muscle 645546 2.8.1.1 0.026 - abomasum 645546 2.8.1.1 0.027 - enzyme from lung, pH 9.2, 37°C 673175 2.8.1.1 0.027 - kidney 645546 2.8.1.1 0.029 - rumen 645546 2.8.1.1 0.03 - enzyme from lung, pH 9.2, 37°C 673175 2.8.1.1 0.033 - rumen 645546 2.8.1.1 0.035 - enzyme from brain, pH 9.2, 37°C 673175 2.8.1.1 0.04 - enzyme from muscle, pH 9.2, 37°C 673175 2.8.1.1 0.04 - enzyme from stomach, pH 9.2, 37°C 673175 2.8.1.1 0.04 - rhodanese activity in spleen of male and female animals 645530 2.8.1.1 0.046 - omasum 645546 2.8.1.1 0.047 - oesophagus 645535 2.8.1.1 0.05 - enzyme from kidney medula, pH 9.2, 37°C 673175 2.8.1.1 0.05 - enzyme from muscle, pH 9.2, 37°C 673175 2.8.1.1 0.05 - enzyme from muscular layer of rumen, pH 9.2, 37°C 673175 2.8.1.1 0.05 - rhodanese activity in urinary bladder of male animals 645530 2.8.1.1 0.06 - enzyme from kidney, pH 9.2, 37°C 673175 2.8.1.1 0.06 - rhodanese activity in urinary bladder of female animals 645530 2.8.1.1 0.069 - rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of methionine 645534 2.8.1.1 0.07 - enzyme from stomach, pH 9.2, 37°C 673175 2.8.1.1 0.07 - rhodanese activity in lymph node of male and female animals 645530 2.8.1.1 0.07 - rhodanese activity in ovary of female animals 645530 2.8.1.1 0.071 - rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of 0.9% NaCl as control 645534 2.8.1.1 0.071 - rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of thiosulfate 645534 2.8.1.1 0.077 - rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of cysteine 645534 2.8.1.1 0.078 - rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of thiocystine 645534 2.8.1.1 0.08 - enzyme from kidney, pH 9.2, 37°C 673175 2.8.1.1 0.08 - enzyme from muscle, pH 9.2, 37°C 673175 2.8.1.1 0.08 - rhodanese activity in pylore muscle of abomasum of male animals 645530 2.8.1.1 0.08 - rhodanese activity in skeletal muscle of male and female animals 645530 2.8.1.1 0.085 - rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of 2-methyl-thiazolidine-4-carboxylic acid 645534 2.8.1.1 0.09 - enzyme from muscular layer of rumen, pH 9.2, 37°C 673175 2.8.1.1 0.09 - enzyme from stomach, pH 9.2, 37°C 673175 2.8.1.1 0.09 - microsomal rhodanese 42 days after common bile duct ligation 645540 2.8.1.1 0.09 - rhodanese activity in pylore muscle of abomasum of female animals 645530 2.8.1.1 0.09 - rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of thiazolidine-4-carboxylic acid 645534 2.8.1.1 0.092 - kidney 645546 2.8.1.1 0.1 - microsomal rhodanese 28 days after common bile duct ligation 645540 2.8.1.1 0.11 - rhodanese activity in muscle of omasum of male and female animals 645530 2.8.1.1 0.113 - rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of 2-methyl-thiazolidine-2,4-dicarboxylic acid 645534 2.8.1.1 0.12 - microsomal rhodanese 14 days after common bile duct ligation 645540 2.8.1.1 0.12 - rhodanese activity in muscle of rumen of male animals 645530 2.8.1.1 0.128 - stomach 645535 2.8.1.1 0.13 - enzyme from brain, pH 9.2, 37°C 673175 2.8.1.1 0.13 - enzyme from lung, pH 9.2, 37°C 673175 2.8.1.1 0.13 - rhodanese activity in muscle of rumen of female animals 645530 2.8.1.1 0.13 - rhodanese activity in prostate of male animals 645530 2.8.1.1 0.15 - enzyme from brain, pH 9.2, 37°C 673175 2.8.1.1 0.15 - enzyme from liver, pH 9.2, 37°C 673175 2.8.1.1 0.15 - enzyme from muscle, pH 9.2, 37°C 673175 2.8.1.1 0.158 - small intestine 645535 2.8.1.1 0.159 - microsomal rhodanese 28 days after common bile duct ligation 645540 2.8.1.1 0.18 - enzyme from liver, pH 9.2, 37°C 673175 2.8.1.1 0.18 - rhodanese activity in muscle of reticulum of rumen of male animals 645530 2.8.1.1 0.183 - liver 645546 2.8.1.1 0.19 - rhodanese activity in muscle of reticulum of rumen of female animals 645530 2.8.1.1 0.21 - rhodanese activity in fundus muscle of abomasum of male animals 645530 2.8.1.1 0.214 - liver 645546 2.8.1.1 0.22 - rhodanese activity in fundus muscle of abomasum of female animals 645530 2.8.1.1 0.23 - rhodanese activity in the liver of tumor-bearing mouse on the fourth day after intraperitoneal administration of cysteine 645534 2.8.1.1 0.24 - enzyme from kidney medula, pH 9.2, 37°C 673175 2.8.1.1 0.24 - enzyme from lung, pH 9.2, 37°C 673175 2.8.1.1 0.24 - microsomal rhodanese 12 h, 14, 28 and 24 days after apparent operation 645540 2.8.1.1 0.24 - rhodanese activity in kidney medulla of male animals 645530 2.8.1.1 0.246 - left colon 645535 2.8.1.1 0.25 - enzyme from kidney medula, pH 9.2, 37°C 673175 2.8.1.1 0.25 - microsomal rhodanese 1, 2,3 and 7 days after apparent operation 645540 2.8.1.1 0.25 - microsomal rhodanese 7 days after common bile duct ligation 645540 2.8.1.1 0.25 - rhodanese activity in kidney medulla of female animals 645530 2.8.1.1 0.254 - rectum 645535 2.8.1.1 0.26 - microsomal rhodanese 12 h after common bile duct ligation 645540 2.8.1.1 0.26 - substrate H2S, pH 9.2, 22°C 673274 2.8.1.1 0.28 - enzyme from rumen, pH 9.2, 37°C 673175 2.8.1.1 0.28 - rhodanese activity in small intestine of male animals 645530 2.8.1.1 0.289 - microsomal rhodanese 28 days after apparent operation 645540 2.8.1.1 0.29 - rhodanese activity in small intestine of female animals 645530 2.8.1.1 0.292 - right colon 645535 2.8.1.1 0.3 - rhodanese activity in heart ventricle of female animals 645530 2.8.1.1 0.31 - enzyme from liver, pH 9.2, 37°C 673175 2.8.1.1 0.31 - microsomal rhodanese 2 and 3 days after common bile duct ligation 645540 2.8.1.1 0.31 - rhodanese activity in pylore of abomasum of male and female animals 645530 2.8.1.1 0.314 - liver 645546 2.8.1.1 0.32 - enzyme from brain, pH 9.2, 37°C 673175 2.8.1.1 0.32 - muscle 645546 2.8.1.1 0.34 - rhodanese activity in heart ventricle of male animals 645530 2.8.1.1 0.35 - enzyme from stomach, pH 9.2, 37°C 673175 2.8.1.1 0.35 - microsomal rhodanese 1 day after common bile duct ligation 645540 2.8.1.1 0.35 - rhodanese activity in fundus of abomasum of male animals 645530 2.8.1.1 0.36 - enzyme from brain, pH 9.2, 37°C 673175 2.8.1.1 0.36 - enzyme from lung, pH 9.2, 37°C 673175 2.8.1.1 0.36 - rhodanese activity in fundus of abomasum of female animals 645530 2.8.1.1 0.36 - rhodanese activity in lung of male animals 645530 2.8.1.1 0.36 - rhodanese activity in the liver of tumor-bearing mouse on the fourth day after intraperitoneal administration of thiosulfate 645534 2.8.1.1 0.362 - rhodanese activity in the liver of tumor-bearing mouse on the fourth day after intraperitoneal administration of 2-methyl-thiazolidine-2,4-dicarboxylic acid 645534 2.8.1.1 0.37 - rhodanese activity in lung of female animals 645530 2.8.1.1 0.37 - rhodanese activity in the liver of tumor-bearing mouse on the fourth day after intraperitoneal administration of 0.9% NaCl as control 645534 2.8.1.1 0.374 - rhodanese activity in the liver of tumor-bearing mouse on the fourth day after intraperitoneal administration of 2-methyl-thiazolidine-4-carboxylic acid 645534 2.8.1.1 0.376 - rhodanese activity in the liver of tumor-bearing mouse on the fourth day after intraperitoneal administration of methionine 645534 2.8.1.1 0.38 - rhodanese activity in heart atrium of male animals 645530 2.8.1.1 0.39 - rhodanese activity in heart atrium of female animals 645530 2.8.1.1 0.4 - rhodanese activity in the liver of tumor-bearing mouse on the fourth day after intraperitoneal administration of thiocystine 645534 2.8.1.1 0.426 - kidney 645546 2.8.1.1 0.47 - enzyme from brain, pH 9.2, 37°C 673175 2.8.1.1 0.47 - rhodanese activity in brain of male animals 645530 2.8.1.1 0.476 - rhodanese activity in the liver of tumor-bearing mouse on the fourth day after intraperitoneal administration of thiazolidine-4-carboxylic acid 645534 2.8.1.1 0.48 - enzyme from brain, pH 9.2, 37°C 673175 2.8.1.1 0.49 - rhodanese activity in brain of female animals 645530 2.8.1.1 0.51 - rhodanese activity in fundus epithelium of abomasum of male animals 645530 2.8.1.1 0.52 - rhodanese activity in fundus epithelium of abomasum of female animals 645530 2.8.1.1 0.56 - rhodanese activity in pylore epithelium of abomasum of male animals 645530 2.8.1.1 0.56 - spleen 645546 2.8.1.1 0.57 - rhodanese activity in pylore epithelium of abomasum of female animals 645530 2.8.1.1 0.59 - muscle 645546 2.8.1.1 0.6 - rhodanese activity in large intestine of male animals 645530 2.8.1.1 0.619 - kidney 645546 2.8.1.1 0.62 - rhodanese activity in large intestine of male animals 645530 2.8.1.1 0.63 - preparative PAGE 645512 2.8.1.1 0.67 - spleen 645546 2.8.1.1 0.82 - enzyme from kidney cortex, pH 9.2, 37°C 673175 2.8.1.1 0.88 - kidney 645546 2.8.1.1 0.903 - liver 645546 2.8.1.1 0.94 - enzyme from kidney cortex, pH 9.2, 37°C 673175 2.8.1.1 0.94 - rhodanese activity in kidney cortex of male animals 645530 2.8.1.1 0.965 - enzyme from kidney, pH 9.2, 37°C 673175 2.8.1.1 0.97 - rhodanese activity in kidney cortex of female animals 645530 2.8.1.1 1.03 - cytosolic rhodanese 42 days after common bile duct ligation 645540 2.8.1.1 1.06 - cytosolic rhodanese 28 days after common bile duct ligation 645540 2.8.1.1 1.08 - mitochondrial rhodanese 42 days after common bile duct ligation 645540 2.8.1.1 1.11 - cytosolic rhodanese 14 days after common bile duct ligation 645540 2.8.1.1 1.14 - mitochondrial rhodanese 28 days after common bile duct ligation 645540 2.8.1.1 1.2 - - 645500 2.8.1.1 1.25 - liver 645546 2.8.1.1 1.35 - cytosolic rhodanese 28 days after common bile duct ligation 645540 2.8.1.1 1.4 - enzyme from kidney, pH 9.2, 37°C 673175 2.8.1.1 1.68 - mitochondrial rhodanese 28 days after common bile duct ligation 645540 2.8.1.1 1.7 - enzyme from kidney cortex, pH 9.2, 37°C 673175 2.8.1.1 1.7 - enzyme from liver, pH 9.2, 37°C 673175 2.8.1.1 1.83 - cytosolic rhodanese 7 days after common bile duct ligation 645540 2.8.1.1 2.18 - mitochondrial rhodanese 14 days after common bile duct ligation 645540 2.8.1.1 2.27 - cytosolic rhodanese 3 days after common bile duct ligation 645540 2.8.1.1 2.44 - mitochondrial rhodanese 7 days after common bile duct ligation 645540 2.8.1.1 2.5 - cytosolic rhodanese 2 days after common bile duct ligation 645540 2.8.1.1 2.6 - pH 7.4, 37°C 761486 2.8.1.1 2.61 - cytosolic rhodanese 1 day after common bile duct ligation 645540 2.8.1.1 2.85 - cytosolic rhodanese 12 h after common bile duct ligation 645540 2.8.1.1 2.93 - enzyme from liver, pH 9.2, 37°C 673175 2.8.1.1 2.93 - rhodanese activity in liver of male animals 645530 2.8.1.1 2.99 - rhodanese activity in omasum of male animals 645530 2.8.1.1 3 - rhodanese activity in liver of female animals 645530 2.8.1.1 3 - rhodanese activity in omasum of female animals 645530 2.8.1.1 3.06 - cytosolic rhodanese 12 h after apparent operation 645540 2.8.1.1 3.07 - cytosolic rhodanese 14 and 42 days after apparent operation 645540 2.8.1.1 3.08 - cytosolic rhodanese 3 days after apparent operation 645540 2.8.1.1 3.09 - cytosolic rhodanese 7 and 28 days after apparent operation 645540 2.8.1.1 3.1 - cytosolic rhodanese 1 and 2 days after apparent operation 645540 2.8.1.1 3.14 - rhodanese activity in reticulum of rumen of female animals 645530 2.8.1.1 3.25 - mitochondrial rhodanese 3 days after common bile duct ligation 645540 2.8.1.1 3.31 - enzyme from epithelial layer of rumen, pH 9.2, 37°C 673175 2.8.1.1 3.31 - rhodanese activity in reticulum of rumen of male animals 645530 2.8.1.1 3.63 - cytosolic rhodanese 28 days after apparent operation 645540 2.8.1.1 3.76 - mitochondrial rhodanese 2 days after common bile duct ligation 645540 2.8.1.1 3.76 - rhodanese activity in epithelium of omasum of male animals 645530 2.8.1.1 3.82 - rhodanese activity in epithelium of omasum of female animals 645530 2.8.1.1 4.02 - mitochondrial rhodanese 1 day after common bile duct ligation 645540 2.8.1.1 4.16 - rhodanese activity in rumen of male animals 645530 2.8.1.1 4.18 - mitochondrial rhodanese 12 h after common bile duct ligation 645540 2.8.1.1 4.23 - rhodanese activity in rumen of female animals 645530 2.8.1.1 4.25 - mitochondrial rhodanese 7 and 42 days after apparent operation 645540 2.8.1.1 4.26 - mitochondrial rhodanese 14 and 28 days after apparent operation 645540 2.8.1.1 4.3 - mitochondrial rhodanese 2 and 3 days after apparent operation 645540 2.8.1.1 4.31 - mitochondrial rhodanese 1 day after apparent operation 645540 2.8.1.1 4.35 - mitochondrial rhodanese 12 h after apparent operation 645540 2.8.1.1 4.64 - mitochondrial rhodanese 28 days after apparent operation 645540 2.8.1.1 4.95 - enzyme from liver, pH 9.2, 37°C 673175 2.8.1.1 5 - rhodanese activity in epithelium of reticulum of rumen of female animals 645530 2.8.1.1 5.08 - rhodanese activity in epithelium of reticulum of rumen of male animals 645530 2.8.1.1 5.21 - enzyme from liver, pH 9.2, 37°C 673175 2.8.1.1 6 - - 645500 2.8.1.1 8.92 - rhodanese activity in epithelium of rumen of female animals 645530 2.8.1.1 8.99 - rhodanese activity in epithelium of rumen of male animals 645530 2.8.1.1 10.3 - enzyme from epithelial layer of rumen, pH 9.2, 37°C 673175 2.8.1.1 15.9 - Sephadex G-100 645513 2.8.1.1 16.3 - enzyme from epithelial layer of rumen, pH 9.2, 37°C 673175 2.8.1.1 21 - - 645532 2.8.1.1 70 - QAE-Sephadex column II 645515 2.8.1.1 85.3 - substrate thiosulfate, pH 9.2, 22°C 673274 2.8.1.1 225 - recombinant wild-type enzyme 645522 2.8.1.1 242 - - 645499 2.8.1.1 297.9 - - 645497 2.8.1.1 332 - wild-type 645529 2.8.1.1 353 - Ser-1 645529 2.8.1.1 389 - Ser-2 645529 2.8.1.1 391 - one additional Arg residue at the 3'-end followed by a TAA stop codon 645529 2.8.1.1 440 - T232A 645522 2.8.1.1 491 - C-terminally extended mutant enzyme 645531 2.8.1.1 550 - T232K 645522 2.8.1.1 575.5 - R186L 645527 2.8.1.1 600 - main fraction of Sephadex G-200 gel chromatography 645511 2.8.1.1 672.8 - wild-type 645527 2.8.1.1 700 - preparative polyacrylamide gel electrophoresis 645518 2.8.1.1 720 - - 645503 2.8.1.1 730 - purificated liver rhodanese 645508 2.8.1.1 816 - pH 7.3, 25°C 661492 2.8.1.1 816 - washed precipitate of recombinant rhodanese 645508 2.8.1.1 818 - recombinant wild-type 645531 2.8.1.1 850 - pH 9.0, 80°C 673600 2.8.1.1 1071 - after Sephacryl S-200-HR gel filtration 645536