1.1.1.71 additional information - specific activities of enzyme mutants with the different substrates, overview 760769 1.1.1.71 0.01 - with acetone, at pH 7.5 and 30°C 740200 1.1.1.71 0.025 - wild-type, cofoactor NADPH, pH 7.6, 55°C 739526 1.1.1.71 0.05 - with acetaldehyde, at pH 7.5 and 30°C 740200 1.1.1.71 0.052 - mutant P704L/H734R, cofoactor NADH, pH 7.6, 55°C 739526 1.1.1.71 0.08 - with 2,5-hexandione, at pH 7.5 and 30°C 740200 1.1.1.71 0.11 - with cyclohexanone, at pH 7.5 and 30°C 740200 1.1.1.71 0.12 - mutant P704L/H734R, cofoactor NADPH, pH 7.6, 55°C 739526 1.1.1.71 0.33 - wild-type enzyme, pH 6.5, 28°C, substrate (4R,6S)-6-(chloromethyl)oxane-2,4-diol 760769 1.1.1.71 0.56 - with 4-phenyl-2-butanone, at pH 7.5 and 30°C 740200 1.1.1.71 2.7 - wild-type, cofoactor NADH, pH 7.6, 55°C 739526 1.1.1.71 4.11 - wild-type enzyme, pH 7.0, 28°C, substrate ethyl acetoacetate 760769 1.1.1.71 4.11 - wild-type enzyme, pH 7.5, 28°C, substrate (4R,6S)-6-(chloromethyl)oxane-2,4-diol 760769 1.1.1.71 4.35 - with acetophenone, at pH 7.5 and 30°C 740200 1.1.1.71 4.89 - with benzaldehyde, at pH 7.5 and 30°C 740200 1.1.1.71 8.36 - with cyclohexanol, at pH 10.5 and 30°C 740200 1.1.1.71 9.78 - with cyclohexanone, at pH 7.5 and 30°C 740200 1.1.1.71 11.58 - with 3-methylcyclohexanol, at pH 10.5 and 30°C 740200 1.1.1.71 11.7 - with 3-fluorobenzaldehyde, at pH 7.5 and 30°C 740200 1.1.1.71 12.62 - with 3-methylcyclohexanone, at pH 7.5 and 30°C 740200 1.1.1.71 12.9 - wild-type enzyme, pH 7.0, 28°C, substrate (4R,6R)-6-butyloxane-2,4-diol 760769 1.1.1.71 17.1 - with (S)-2-hydroxypropiophenone, at pH 7.5 and 30°C 740200 1.1.1.71 18.83 - with cyclohexenol, at pH 10.5 and 30°C 740200 1.1.1.71 23.31 - 2-bromobenzaldehyde, at pH 7.5 and 30°C 740200 1.1.1.71 362.6 - with (R)-2-hydroxypropiophenone, at pH 7.5 and 30°C 740200