1.1.1.67 additional information - significant yield of D-mannitol (approximately 40%) from 1% w/v D-fructose by Thermotoga neapolitana MtDH 763052 1.1.1.67 additional information - the activity for inositol, sorbitol, galactitol, ribitol, xylitol, arabitol and erythritol is very low 668450 1.1.1.67 0.003 - cells grown on D-glucose, crude extracts 287240 1.1.1.67 0.012 - cells grown on D-mannitol + glucose, crude extracts 287240 1.1.1.67 0.015 - cells grown on D-ribose, crude extracts 287240 1.1.1.67 0.05 - 41°C, pH 9.0 94720 1.1.1.67 0.243 - cells grown on D-mannitol, crude extracts 287240 1.1.1.67 6.9 - - 285866 1.1.1.67 10.24 - - 287251 1.1.1.67 14 - crude extract, at 25°C, using 100 mM Tris-HCl buffer, pH 7.1 697276 1.1.1.67 15.48 - - 655617 1.1.1.67 27.3 - - 287257 1.1.1.67 31 - - 287262 1.1.1.67 33 - after 6.7fold purification, at 25°C, using 100 mM Tris-HCl buffer, pH 7.1 697276 1.1.1.67 46 - histidine-tagged recombinant protein 287262 1.1.1.67 54 - purified enzyme at 80°C with D-fructose as the substrate and NADH as the cofactor 695814 1.1.1.67 54 - purified enzyme, at 80°C with fructose as the substrate and NADH as the cofactor 695814 1.1.1.67 60.3 - - 287237 1.1.1.67 63 - MDH expressed in Escherichia coli strain BL21 (DE3) plysS, with fructose as substrate 700553 1.1.1.67 68.3 - recombinant protein 287259 1.1.1.67 393 - - 287252 1.1.1.67 450 - - 654390