3.6.1.25 additional information - tripolyphosphate activity of R265H mutant is specific for tripolyphosphate and depends on magnesium ions, potassium is not required 669193 3.6.1.25 0.000006 - substrate ATP, formation of cAMP, presence of Mn2+, Mg2+, 30°C, pH 7.5 771102 3.6.1.25 0.001 - R265S mutant, 2 mM methionine and 2 mM ATP as substrate 669193 3.6.1.25 0.0012 - tripolyphosphatase activity of R265H mutant, 2 mM ATP as substrate 669193 3.6.1.25 0.0019 - tripolyphosphatase activity of R265H mutant, 2 mM pyrophosphate as substrate 669193 3.6.1.25 0.002 - R265H mutant, 2 mM methionine and 2 mM ATP as substrate 669193 3.6.1.25 0.004 - tripolyphosphatase activity of R265H mutant, 2 mM metatripolyphosphate as substrate 669193 3.6.1.25 0.007 - substrate ATP, release of ADP and phosphate, presence of Mn2+, Mg2+, 30°C, pH 7.5 771102 3.6.1.25 0.0115 - substrate triphosphate, presence of Mn2+, Mg2+, 30°C, pH 7.5 771102 3.6.1.25 0.029 - tripolyphosphatase activity of R265S mutant, 2 mM tripolyphosphate as substrate 669193 3.6.1.25 0.067 - tripolyphosphatase activity of R265H mutant, 2 mM tripolyphosphate as substrate in the presence of 5 mM pyrophosphate 669193 3.6.1.25 0.128 - tripolyphosphatase activity of R265H mutant, 2 mM tripolyphosphate as substrate 669193 3.6.1.25 0.129 - tripolyphosphatase activity of R265H mutant, 2 mM tripolyphosphate as substrate in the presence of 5 mM ATP 669193 3.6.1.25 0.156 - tripolyphosphatase activity of wildtype, 2 mM tripolyphosphate as substrate 669193 3.6.1.25 0.588 - wildtype, 2 mM methionine and 2 mM ATP as substrate 669193