6.2.1.45 flower bud - 1111, 733931 6.2.1.45 leaf - 1111 6.2.1.45 root - 1111 6.2.1.45 silique - 1111 6.2.1.45 stem - 1111 6.2.1.45 additional information isoform UBA1 mRNA is detected in all tissues analyzed 1115 6.2.1.45 2B4-T cell - 672922 6.2.1.45 A-9 cell - 672922 6.2.1.45 HEK-293 Tet-On 3G cell - 672922 6.2.1.45 JURKAT cell - 672922 6.2.1.45 L-929 cell - 672922 6.2.1.45 SW-60 cell colon carcinoma cell 674842 6.2.1.45 testis most abundant expression 674842 6.2.1.45 testis the UBE1L2 mRNA is most abundantly expressed in the testis 674842 6.2.1.45 HeLa cell - 672922, 676827 6.2.1.45 eye - 692413 6.2.1.45 brain fetal brain 693313 6.2.1.45 kidney embryonic kidney 693313 6.2.1.45 liver - 693313, 725950 6.2.1.45 pancreas - 693313 6.2.1.45 leukemia cell - 707854 6.2.1.45 additional information primary leukemia and multiple myolema patient samples 707854 6.2.1.45 A31N-ts20 cell mouse embryo fibroblast cell, thermosensitive for ubiquitin-activating enzyme E1 727412, 728028 6.2.1.45 erythrocyte - 727814 6.2.1.45 cyst the enzyme localizes at spots within the cytoplasm. This pattern changes during encystation into a diffuse localization throughout the cytoplasm of encysting cells. E1 localizes in mature cysts at cytoplasmic spots and in the cyst wall -, 733043 6.2.1.45 additional information the enzyme expression (both at mRNA and protein levels) is regulated during encystation -, 733043 6.2.1.45 trophozoite E1 localizes in the trophozoites in a precise pattern bound to small granules or vesicles -, 733043 6.2.1.45 anther expression level of the CrUBE1 gene in anthers of cv. Shatangju is approximately 10fold higher than in anthers of cv. Wuzishatangju 733931 6.2.1.45 carpel high enzyme expression level 733931 6.2.1.45 filament very low expression 733931 6.2.1.45 leaf very low expression 733931 6.2.1.45 leaf bud - 733931 6.2.1.45 additional information semi-quantitative and quantitative expressio analysis of the enzyme in different tissues of the two cultivars, o verview, the highest expression level of CrUBE1 is detected in pistils at 7 days after self pollination of cv.Wuzishatangju, which is approximately 5fold higher than at the starting point 733931 6.2.1.45 petal very low expression 733931 6.2.1.45 plant ovary - 733931 6.2.1.45 stigma - 733931 6.2.1.45 style very low expression 733931 6.2.1.45 SK6 cell - 734563 6.2.1.45 HEK-293 cell - 735013 6.2.1.45 brain - 735079 6.2.1.45 epithelial cell - 744237, 745863, 745952 6.2.1.45 K-562 cell - 744237 6.2.1.45 MDA-MB-231 cell - 744237 6.2.1.45 mammary gland - 745863 6.2.1.45 PC-3M cell - 745863 6.2.1.45 prostate - 745863 6.2.1.45 prostate cancer cell - 745863 6.2.1.45 RWPE-1 cell - 745863 6.2.1.45 MCF-10A cell - 745863, 745952 6.2.1.45 thymus ovine thymuses are sampled on day 16 of the estrous cycle and on days 13, 16 and 25 of gestation, and UBE1L is detected by real-time quantitative PCR, Western blot and immunohistochemistry. The expression of UBE1L is declined during early pregnancy 760342 6.2.1.45 additional information the enzyme (UBA1) exhibits ubiquitous expression throughout all human tissues 766918