3.3.2.6 1.497 - L-alanine-7-amido-4-methylcoumarin in 250 mM Tris buffer, pH 7.5, containing 500 mM KCl and 0.1% (w/v) of bovine serum albumin, at 37°C 731116 3.3.2.6 1.7 - L-alanine-4-nitroanilide wild type enzyme, at pH 7.8 and 22°C 731360 3.3.2.6 3.96 - L-alanine-4-nitroanilide mutant enzyme F375Y, at pH 7.8 and 22°C 731360