1.1.1.B4 0.018 - 2'-methylacetophenone pH 6.5, 50°C, wild-type enzyme 741784 1.1.1.B4 0.025 - 3'-trifluoromethyl-acetophenone pH 6.5, 50°C, wild-type enzyme 741784 1.1.1.B4 0.16 - 3'-iodo-acetophenone pH 6.5, 50°C, wild-type enzyme 741784 1.1.1.B4 0.18 - 3'-methylacetophenone pH 6.5, 50°C, wild-type enzyme 741784 1.1.1.B4 0.2 - 1-(3-pyridyl)acetone pH 6.5, 50°C, mutant I86A 741784 1.1.1.B4 0.21 - 3'-methoxy-acetophenone pH 6.5, 50°C, wild-type enzyme 741784 1.1.1.B4 0.23 - 2',4'-difluoro-acetophenone pH 6.5, 50°C, wild-type enzyme 741784 1.1.1.B4 0.26 - 1-(2-pyridyl)acetone pH 6.5, 50°C, wild-type enzyme 741784 1.1.1.B4 0.26 - 1-(2-thienyl)acetone pH 6.5, 50°C, wild-type enzyme 741784 1.1.1.B4 0.35 - 1-(2-thienyl)acetone pH 6.5, 50°C, wild-type enzyme 741784 1.1.1.B4 0.41 - 3'-chloro-acetophenone pH 6.5, 50°C, mutant I86A 741784 1.1.1.B4 0.51 - 1-(2-thienyl)acetone pH 6.5, 50°C, mutant I86A 741784 1.1.1.B4 0.62 - 2',4'-difluoro-acetophenone pH 6.5, 50°C, mutant I86A 741784 1.1.1.B4 0.63 - 1-(3-pyridyl)acetone pH 6.5, 50°C, wild-type enzyme 741784 1.1.1.B4 0.65 - 3'-bromo-acetophenone pH 6.5, 50°C, wild-type enzyme 741784 1.1.1.B4 1.05 - 1-(4-pyridyl)acetone pH 6.5, 50°C, mutant I86A 741784 1.1.1.B4 1.08 - acetone pH 6.5, 50°C, wild-type enzyme 741784 1.1.1.B4 1.3 - 3'-chloro-acetophenone pH 6.5, 50°C, wild-type enzyme 741784 1.1.1.B4 1.33 - acetone pH 6.5, 50°C, mutant I86A 741784 1.1.1.B4 1.5 - 1-phenylpropanone pH 6.5, 50°C, wild-type enzyme 741784 1.1.1.B4 1.6 - 1-(2-pyridyl)acetone pH 6.5, 50°C, mutant I86A 741784 1.1.1.B4 1.9 - acetophenone pH 6.5, 50°C, wild-type enzyme 741784 1.1.1.B4 2.4 - 1-(4-pyridyl)acetone pH 6.5, 50°C, wild-type enzyme 741784 1.1.1.B4 4.1 - ethyl 4-chloro-3-oxobutanoate pH 7.4, 30°C 740394 1.1.1.B4 4.3 - 1-(3-thienyl)acetone pH 6.5, 50°C, mutant I86A 741784 1.1.1.B4 8.1 - 1-phenylpropanone pH 6.5, 50°C, mutant I86A 741784 1.1.1.B4 14.9 - acetophenone pH 6.5, 50°C, mutant I86A 741784 1.1.1.B4 36.8 - 3,5-bis(trifluoromethyl)acetophenone wild-type, pH 7.2, 30°C 739942 1.1.1.B4 50.1 - 2,3-Butanedione pH 7.4, 30°C 740394 1.1.1.B4 75 - NADH wild-type, pH 6.5, 30°C 711860 1.1.1.B4 277 - 3,5-bis(trifluoromethyl)acetophenone mutant S154Y/L194I, pH 7.2, 30°C 739942 1.1.1.B4 300 - NADH mutant R38P, pH 5.5, 30°C 711860 1.1.1.B4 463 - 3,5-bis(trifluoromethyl)acetophenone mutant S154Y, pH 7.2, 30°C 739942 1.1.1.B4 944 - NADH wild-type, pH 7.2, 30°C 739942 1.1.1.B4 3846 - NADH mutant S154Y/L194I, pH 7.2, 30°C 739942 1.1.1.B4 4207 - NADH mutant S154Y, pH 7.2, 30°C 739942 1.1.1.B4 5100 - NADPH mutant R38P, pH 5.5, 30°C 711860 1.1.1.B4 9300 - NADPH wild-type, pH 6.5, 30°C 711860