2.6.1.97	evolution	specific Archaea such as Sulfolobus tokodaii have retained ArcS in addition to GAT-QueC, overview	717018	
2.6.1.97	evolution	structure-based alignments comparing arcTGT and TgtA2 reveal that TgtA2 lacks key arcTGT catalytic residues and contains an additional module. Members of the TgtA2 and arcTGT family do not perfectly co-distribute	-, 719859	
2.6.1.97	evolution	the hyperthermophilic euryarchaeon Thermococcus kodakarensis lacks an arcTGT orthologue	-, 759819	
2.6.1.97	malfunction	a Haloferax volcanii DELTAtgtA2 derivative demonstrates that tRNA from the mutant strain lacks G+ and instead accumulates preQ0	-, 719859	
2.6.1.97	malfunction	recombinant expression of the arcTGT orthologue from Thermoplasma acidophilum in the hyperthermophilic euryarchaeon Thermococcus kodakarensis arcS-deletion strain and functional complementation. Less TkRaSEA is obtained from the extract containing TaArcS compared to TkArcS	-, 759819	
2.6.1.97	metabolism	archaeosine biosynthesis pathway, overview	-, 759819	
2.6.1.97	metabolism	GAT-QueC also catalyzes biosynthesis of G+-tRNA, pathways, overview	717018	
2.6.1.97	metabolism	TgtA2 is involved in archaeosine biosynthesis in vivo. Archaeosine biosynthesis is especially complex, involving the initial production of 7-cyano-7-deazaguanine (preQ0), an advanced precursor that is produced in a tRNA-independent portion of the biosynthesis, followed by its insertion into the tRNA by the enzyme tRNA-guanine transglycosylase, which replaces the target guanine base yielding preQ0-tRNA	-, 719859	
2.6.1.97	metabolism	the enzyme is responsible for the final step in the biosynthesis of archaeosine in the D-loop of tRNA	-, 765740	
2.6.1.97	additional information	G+ can be tolerated in Escherichia coli at position 34 in normally Q-containing tRNA when recombinant GAT-QueC and QueF are introduced	717018