2.4.1.309 malfunction wbnI gene is replaced by a chloramphenicol acetyltransferase gene using the RED recombination system of phage lambda. Inactivated the wbnI gene shows that the mutant strain produces a different polysaccharide without the a-(1->3)-linked Galp side chain. The yield of the polysaccharide is substantially lower than the one produced by the wild-type strain -, 721974 2.4.1.309 physiological function the enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86 -, 723245