2.4.1.14 evolution a comparison of the sequences of the wheat SPSII orthologues present in the diploid progenitors Triticum monococcum, Triticum urartu, Triticum speltoides, Aegilops tauschii, and Triticum speltoides, as well as in the more distantly related species Hordeum vulgare, Oryza sativa, Sorghum and purple false brome, Brachypodium distachyon, demonstrates that intronic sequence is less well conserved than exonic. Comparative sequence and phylogenetic analysis of SPSII gene shows that false purple brome is more similar to Triticeae than to Oryza sativa 719182 2.4.1.14 malfunction an spsa1 knock-out mutant shows a 44% decrease in leaf enzyme activity compared to the wild-type and a slight increase in leaf starch content at the end of the light period as well as at the end of the dark period. An spsa1/spsc double mutant is strongly impaired in growth and accumulated high levels of starch. This increase in starch is probably not due to an increased partitioning of carbon into starch, but is rather caused by an impaired starch mobilization during the night. Sucrose export from excised petioles harvested from spsa1/spsc double mutant plants is significantly reduced under illumination as well as during the dark period. Loss of the two major SPS isoforms in leaves limits Suc synthesis without grossly changingcarbon partitioning in favour of starch during the light period but limits starch degradation during the dark period 736569 2.4.1.14 malfunction knockdown and knockout mutants of isoform SPS1 show a 29-46% reduction in enzyme activity in the leaves, but the carbohydrate content in the leaves and plant growth are not significantly different from those of wild type plants. In a double knockout mutant of SPS1 and SPS11, an 84% reduction in leaf enzyme activity results in higher starch accumulation in the leaves than in the wild type leaves. However, the double knockout mutant plants grow normally 758076 2.4.1.14 malfunction mutation of AtSWEET9 or nectary-expressed sucrose phosphate synthase genes leads to the loss of nectar secretion 736881 2.4.1.14 malfunction overexpression and increased activity of the enzyme in alfalfa is accompanied by early flowering, increased plant growth and an increase in elemental N and protein content when grown under N2-fixing conditions 737054 2.4.1.14 malfunction suppression of isoform SPS4 promotes carbon metabolism to accumulate starch 757497 2.4.1.14 malfunction the spsc null mutant displays reduced sucrose contents towards the end of the photoperiod and a concomitant 25% reduction in enzyme activity. In contrast, an spsa1/spsc double mutant is strongly impaired in growth and accumulated high levels of starch. This increase in starch is probably not due to an increased partitioning of carbon into starch, but is rather caused by an impaired starch mobilization during the night. Sucrose export from excised petioles harvested from spsa1/spsc double mutant plants is significantly reduced under illumination as well as during the dark period. Loss of the two major SPS isoforms in leaves limits Suc synthesis without grossly changing carbon partitioning in favour of starch during the light period but limits starch degradation during the dark period 736569 2.4.1.14 metabolism relationship between the contents of carbohydrate and sucrose metabolizing enzymes activities, overview 736597 2.4.1.14 metabolism relationship between the contents of carbohydrate and sucrose-metabolizing enzymes activities, overview 736597 2.4.1.14 metabolism SPS catalyzes the first step in the synthesis of sucrose in photosynthetic tissue 706394