5.4.99.22 malfunction deletion of rluB leads to accumulation of free 30S and 50S ribosomal subunits. Growth of an rluB deletion mutant is impaired in the cold 704271 5.4.99.22 malfunction no formation of pseudouridine2605 in 23S rRNA of an rluB null mutant. An rluB null mutant or an rluB rluF double null mutant exhibits no obvious growth defect 706734 5.4.99.22 physiological function RluB is associated with a particle that migrates slightly slower than the 50S ribosomal subunit, suggesting that it acts during 50S subunit maturation 704271