2.7.1.186 evolution mevalonate 3-phosphate 5-kinase is an enzyme involved in the modified mevalonate pathway specific to limited species of thermophilic archaea -, 758668 2.7.1.186 metabolism mevalonate 3-kinase plays a key role in a recently discovered modified mevalonate pathway specific to thermophilic archaea of the order Thermoplasmatales, pathway overview. In the pathway called modified MVA pathway II, mevalonate (MVA) is phosphorylated at the 3-hydroxyl group to yield 3-phosphomevalonate (MVA-3-P) by the action of mevalonate 3-kinase (M3K, EC 2.7.1.185) rather than at the 5-hydroxyl group as in the reaction of MVK (EC 2.7.4.2). M3K is also homologous to diphosphomevalonate decarboxylase (DMD). After the formation of MVA-3-P, another kinase, MVA-3-P 5-kinase (M3P5K), catalyzes its 5-phosphorylation, and a subsequent decarboxylation is catalyzed by another DMD homologue, 3,5-bisphosphomevalonate decarboxylase (BMD), to give isopentenyl phosphate (IP). IP is then phosphorylated by isopentenyl phosphate kinase (IPK) to yield isopentenyl diphosphate (IPP) -, 758668 2.7.1.186 physiological function mevalonate 3-phosphate 5-kinase catalyzes the ATP-dependent 5-phosphorylation of 3-phosphomevalonate -, 758668 2.7.1.186 physiological function mevalonate-3-kinase and mevalonate-3-phosphate-5-kinase act sequentially in a putative alternate mevalonate pathway in Thermoplasma acidophilum 727046