2.5.1.147 malfunction four independent mutants that can not make F420 or the biosynthesis intermediate FO contain transposons inserted in the Mycobacterium bovis gene fbiC 748075 2.5.1.147 metabolism the enzyme is essential for F420 production 748075 2.5.1.147 metabolism the enzyme is involved in biosynthesis cofactor coenzyme F420 -, 748607 2.5.1.147 metabolism the enzyme is involved in the production of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO), the precursor of the redox cofactor coenzyme F420 -, 748030 2.5.1.147 metabolism the enzyme is involved in the production of 7,8-didemethyl-8-hydroxy-5-deazariboflavin, the precursor of the redox cofactor coenzyme F420 -, 746934 2.5.1.147 metabolism the enzyme is involved in the production of 7,8-didemethyl-8-hydroxy-5-deazariboflavin, the precursor of the redox cofactor coenzyme F420. In the actinomycetes, cofG and cofH are fused, encoding a single FbiC polypeptide, the bifunctional FO synthase (EC 2.5.1.147/EC 4.3.1.32) 746934