2.4.1.215	malfunction	constitutive expression of OscZOG1 leads to negative effects on the formation of the grain-yielding traits with a marked increase in the accumulation levels of cis-zeatin O-glucoside (cZOG) in the transgenic rice plants. Growth of lateral roots is stimulated in OscZOG1 overexpression lines, but inhibited in OscZOG1 RNA interference lines. Modulation of OscZOG1 expression affects lateral root development in rice. The RNAi transgenic lines exhibit a higher plant height, whereas the plant height of the overexpression lines is reduced with respect to wild-type seedlings when grown in liquid culture conditions in greenhouse for 2 weeks, drastic enhancement of tiller numbers in RNAi transgenic lines, phenotypes, overview	759588	
2.4.1.215	malfunction	cZOGT3 overexpressing lines do not show shortened shoots	723476	
2.4.1.215	malfunction	transgenic rice lines ectopically overexpressing the cZOGT1 gene exhibit short-shoot phenotypes, delay of leaf senescence, and decrease in crown root number	723476	
2.4.1.215	malfunction	transgenic rice lines ectopically overexpressing the cZOGT2 gene exhibit short-shoot phenotypes, delay of leaf senescence, and decrease in crown root number	723476	
2.4.1.215	metabolism	in general, trans-zeatin (tZ) and N6-(DELTA2-isopentenyl)adenine (iP)-types predominate in the spectrum of cytokinins in Brassica napus, with N7-glucosides, namely tZ-N7-glucoside (tZ7G) and iPN7-glucoside (iP7G), representing the most abundant forms. Leptosphaeria maculans is a phytopathogenic fungus. It can produce cytokinins (CKs) in vitro and its CK profile differs from that in tissue of its host Brassica napus. At 7 dpi, CK levels remains statistically unaffected by the infection. With the progression of the infection, the levels of most CK forms increase at 10 dpi. The total CK content increases to 150% compared to mock-infected samples. The highest (240%) increase is observed for cis-zeatin (cZ)-type CKs. All of the detected cZ-type derivatives are induced by infection at 10 dpi, with the free cZ and cZ-N7-glucoside (cZ7G) reaching the highest concentrations. Infection with Leptosphaeria maculans modifies significantly the content of CKs in oilseed rape cotyledon leaves. Cytokinin spectrum and content, overview	759242	
2.4.1.215	metabolism	the fungus Leptosphaeria maculans strain JN3 contains both cis- and trans-zeatin O-glucosyltransferases (EC 2.4.1.215 and EC 2.4.1.203), and a cis-trans-isomerase, that are all involved in the cytokinin (CK) metabolism of the pathogenic fungus. Among the glucosides, glucosides of trans-zeatin (tZ) and N7-glucosides (iP7G) are detected, whereas N9-glucosides are mostly missing in the mycelium. The most abundant metabolite of the tZ-type cytokinins is O-beta-D-glucosyl-trans-zeatin (tZOG). The tZ feeding increases cis-zeatin (cZ). At 9 days, the sum of total CKs in the mycelium increases compared to 7 days, mainly due to the increase of cis-zeatin (cZ)-type CKs, the free cZ especially being the highly predominant CK derivative. Leptosphaeria maculans can produce CKs in vitro and its CK profile differs from that of its host Brassica napus tissue. Leptosphaeria maculans metabolizes exogenously added CKs (iP, tZ, cZ, all at 0.001 mM). Cytokinin spectrum and content, overview. The cis-trans-isomerase performs zeatin cis-trans isomerisation in Leptosphaeria maculans	-, 759242	
2.4.1.215	physiological function	cis-zeatin inhibits seminal root elongation and up-regulates cytokinin-inducible genes, and its activities are comparable to those of trans-zeatin. cis-Zeatin activity has a physiological impact on the growth and development of rice	723476	
2.4.1.215	physiological function	putative zeatin O-glucosyltransferase OscZOG1 regulates root and shoot development and formation of agronomic traits in rice. Cytokinin plays a central role in regulating the activity of the reproductive shoot apical meristem (SAM), which is one parameter determining seed yield in crop plants. OscZOG1 regulates lateral root development. OscZOG1 is highly expressed in shoot meristematic tissues and functions in early seedling growth and tillering. OscZOG1 modulates adult plant height and flag leaf senescence. OscZOG1 regulates panicle development and seed formation	759588