4.2.2.23 malfunction reduced virulence of the rhiE mutant indicates that degradation of the RG-I region of pectin is important for full virulence of Erwinia chrysanthemi 4.2.2.23 physiological function reduced virulence of the rhiE mutant indicates that degradation of the RG-I region of pectin is important for full virulence of Erwinia chrysanthemi 4.2.2.23 physiological function both the full-length Rgl protein (80000 Da) and its truncated derivative (63900 Da) display maximum activity against rhamnogalacturonan I 4.2.2.23 physiological function expression of the Solyc11g011300 gene under a constitutive promoter in tomato cv. Ohio 8245. Transgenic lines exhibit lower rhamnogalacturonan lyase activity in leaves and roots and during fruit ripening, but higher activity is observed at 10, 20, and 30 days after anthesis than in the isogenic line and positive control. Transgenic lines show a lower number of seeds and fruits, higher root length, and less pollen germination percentage and viability. In red ripe tomatoes, transgenic fruits show greater firmness, longer shelf life, and reduced shriveling than wild-type. Additionally, a delay of one week in fruit ripening in transgenic fruits is also recorded 4.2.2.23 physiological function hot buffer-soluble solids from cell wall material of transgenic Solanum tuberosum lines expressing rhamno-galacturonan lyase mutant contain 27% more galacturonic acid and 55% less galactose on fresh potato weight basis, due to the removal of galactan-rich rhamnogalacturonan I segments. All pectin populations of the transgenic strain show consistently low levels of rhamnogalacturonan I segments