2.1.1.241	metabolism	Bx7 catalyzes the last step in benzoxazinoid biosynthesis of maize	
2.1.1.241	physiological function	Bx7 is required for biosynthesis of the benzoxazinoid pesticide 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one, i.e. DIMBOA, that is synthesized in seedlings and stored as glucoside in maize	
2.1.1.241	evolution	gene Bx7 is part of a small gene family consisting of 15 members with higher sequence similarity to each other (62 to 99%), BX7 shows high similarity to BX14	
2.1.1.241	metabolism	the enzyme is involved in benzoxazinoid formation in maize, overview. A 2-oxoglutarate-dependent dioxygenase (BX13, UniProt ID A0A1P8SET2) catalyzes the conversion of 2-(2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one)-beta-D-glucopyranose (DIMBOA-Glc) into a benzoxazinoid intermediate, 2-(2,4,7-trihydroxy-8-methoxy-1,4-benzoxazin-3-one)-beta-D-glucopyranose (TRIMBOA-Glc), by an uncommon reaction involving hydroxylation and a likely ortho-rearrangement of a methoxy group. TRIMBOA-Glc is then converted to 2-(2,4-dihydroxy-7,8-dimethoxy-1,4-benzoxazin-3-one)-beta-D-glucopyranose (DIM2BOA-Glc) by an O-methyltransferase BX7. Furthermore, an O-methyltransferase BX14 (UniProt ID A0A1P8SEU3) converts DIM2BOA-Glc to 2-(2-hydroxy-4,7,8-trimethoxy-1,4-benzoxazin-3-one)-beta-D-glucopyranose (HDM2BOA-Glc). HDM2BOA-Glc is known to be induced by biotic stresses such as pathogen infestation and insect feeding. The inducibility of HDM2BOA-Glc is associated with a stronger induction of genes Bx13 and Bx14. DIM2BOA-Glc and HDM2BOA-Glc specifically increase plant resistance against aphids