3.2.1.125 evolution the enzyme belongs to the glycoside hydrolase family 1, GH1 3.2.1.125 malfunction agrobacterium-mediated transformation on instantaneous expression shows that overexpression of SrRG1 increases the accumulation of steviol content and decreases the major components and total SGs contents. SrRG1 may participate in the steviol glycosides catabolic pathway. However, the effect of silencing construct infiltration on steviol and SGs content is not significant and its expression pattern is constitutive, which most probably, attributed the hydrolysis of SGs to the secondary activity of SrRG1 3.2.1.125 metabolism enzyme SrRG1 may participate in the steviol glycosides catabolic pathway. Total steviol glycosides content is the sum of rebaudioside A (RA), stevioside (St), rebaudioside C (RC), rebaudioside F (RF), and dulcoside A (DA) 3.2.1.125 additional information beta-glucosidase genes from the transcriptome database of Stevia rebaudiana, transcriptome analysis. The enzyme expression pattern analyzed by real-time quantitative PCR shows no significant difference among different tissues, developmental stages, and cultivars under normal growth conditions