2.3.2.23 735257 A disulphide bond in the E2 enzyme Pex4p modulates ubiquitin-conjugating activity Sci. Rep. 3 2212 2013 Saccharomyces cerevisiae https://pubmed.ncbi.nlm.nih.gov/23896733/ 2.3.2.23 736181 A newly discovered ubiquitin-conjugating enzyme E2 correlated with the cryogenic autolysis of Volvariella volvacea Gene 583 58-63 2016 Volvariella volvacea https://pubmed.ncbi.nlm.nih.gov/26927519/ 2.3.2.23 736181 A newly discovered ubiquitin-conjugating enzyme E2 correlated with the cryogenic autolysis of Volvariella volvacea Gene 583 58-63 2016 Volvariella volvacea V23 https://pubmed.ncbi.nlm.nih.gov/26927519/ 2.3.2.23 734357 A specific subset of E2 ubiquitin-conjugating enzymes regulate Parkin activation and mitophagy differently J. Cell Sci. 127 3488-3504 2014 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/24928900/ 2.3.2.23 760168 Active site gate dynamics modulate the catalytic activity of the ubiquitination enzyme E2-25K Sci. Rep. 8 7002 2018 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/29725124/ 2.3.2.23 759600 Allosteric targeting of the Fanconi anemia ubiquitin-conjugating enzyme Ube2T by fragment screening J. Med. Chem. 60 4093-4098 2017 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/28437106/ 2.3.2.23 733621 An E2 enzyme Ubc11 is required for ubiquitination of Slp1/Cdc20 and spindle checkpoint silencing in fission yeast Cell Cycle 12 961-971 2013 Schizosaccharomyces pombe https://pubmed.ncbi.nlm.nih.gov/23442800/ 2.3.2.23 733621 An E2 enzyme Ubc11 is required for ubiquitination of Slp1/Cdc20 and spindle checkpoint silencing in fission yeast Cell Cycle 12 961-971 2013 Schizosaccharomyces pombe 972 https://pubmed.ncbi.nlm.nih.gov/23442800/ 2.3.2.23 779193 An E2 ubiquitin-conjugating enzyme links diubiquitinated H2B to H3K27M oncohistone function Proc. Natl. Acad. Sci. USA 121 e2416614121 2024 Caenorhabditis elegans https://pubmed.ncbi.nlm.nih.gov/39560642/ 2.3.2.23 737253 Biochemical and structural characterization of a novel ubiquitin-conjugating enzyme E2 from Agrocybe aegeria reveals Ube2w family-specific properties Sci. Rep. 5 16056 2015 Cyclocybe aegerita https://pubmed.ncbi.nlm.nih.gov/26525192/ 2.3.2.23 737299 Cellular Ubc2/Rad6 E2 ubiquitin-conjugating enzyme facilitates tombusvirus replication in yeast and plants Virology 484 265-275 2015 Saccharomyces cerevisiae https://pubmed.ncbi.nlm.nih.gov/26135843/ 2.3.2.23 737299 Cellular Ubc2/Rad6 E2 ubiquitin-conjugating enzyme facilitates tombusvirus replication in yeast and plants Virology 484 265-275 2015 Arabidopsis thaliana https://pubmed.ncbi.nlm.nih.gov/26135843/ 2.3.2.23 737299 Cellular Ubc2/Rad6 E2 ubiquitin-conjugating enzyme facilitates tombusvirus replication in yeast and plants Virology 484 265-275 2015 Saccharomyces cerevisiae BY4741 https://pubmed.ncbi.nlm.nih.gov/26135843/ 2.3.2.23 734157 Coactivation of the N-terminal transactivation of mineralocorticoid receptor by Ubc9 J. Biol. Chem. 282 1998-2010 2007 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/17105732/ 2.3.2.23 778238 Comprehensive investigation into the role of ubiquitin-conjugating enzyme E2S in melanoma development J. Invest. Dermatol. 141 374-384 2021 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/32603752/ 2.3.2.23 758487 Conformational dynamics and allostery in E2 E3 interactions drive ubiquitination gp78 and Ube2g2 Structure 25 794-805 2017 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/28434917/ 2.3.2.23 728546 Crystal structure of UBA2ufd-Ubc9: Insights into E1-E2 interactions in Sumo pathways PLoS ONE 1 e15805 2010 Saccharomyces cerevisiae https://pubmed.ncbi.nlm.nih.gov/21209884/ 2.3.2.23 758713 Differential use of E2 ubiquitin conjugating enzymes for regulated degradation of the rate-limiting enzymes HMGCR and SQLE in cholesterol biosynthesis Atherosclerosis 281 137-142 2019 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/30658189/ 2.3.2.23 735196 Distinct activation of an E2 ubiquitin-conjugating enzyme by its cognate E3 ligases Proc. Natl. Acad. Sci. USA 112 E625-E632 2015 Saccharomyces cerevisiae https://pubmed.ncbi.nlm.nih.gov/25646477/ 2.3.2.23 758741 DNA damage-induced foci of E2 ubiquitin-conjugating enzyme are detectable upon co-transfection with an interacting E3 ubiquitin ligase Biochem. Genet. 54 147-157 2016 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/26718580/ 2.3.2.23 727923 E1-E2 interactions in ubiquitin and Nedd8 ligation pathways J. Biol. Chem. 287 311-321 2012 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/22069333/ 2.3.2.23 759030 E2 enzymes more than just middle men Cell Res. 26 423-440 2016 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/27002219/ 2.3.2.23 778854 Echinococcus granulosus ubiquitin-conjugating enzymes (E2D2 and E2N) promote the formation of liver fibrosis in TGFbeta1-induced LX-2 cells Parasit. Vectors 17 190 2024 Echinococcus granulosus https://pubmed.ncbi.nlm.nih.gov/38643149/ 2.3.2.23 778419 Eupalinolide B inhibits periodontitis development by targeting ubiquitin conjugating enzyme UBE2D3 MedComm. (2020) 6 e70034 2025 Mus musculus https://pubmed.ncbi.nlm.nih.gov/39811801/ 2.3.2.23 736156 Evaluation of a SUMO E2 conjugating enzyme involved in resistance to Clavibacter michiganensis subsp. michiganensis in Solanum peruvianum, through a tomato mottle virus VIGS assay Front. Plant Sci. 6 1019 2015 Solanum peruvianum https://pubmed.ncbi.nlm.nih.gov/26734014/ 2.3.2.23 709881 Expression, purification and characterization of human ubiquitin-activating enzyme, UBE1 Mol. Biol. Rep. 37 1413-1419 2010 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/19343538/ 2.3.2.23 777196 Functional conservation and divergence of the helix-turn-helix motif of E2 ubiquitin-conjugating enzymes EMBO J. 41 e108823 2022 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/34942047/ 2.3.2.23 733701 HECT-E3 ligase ETC-1 regulates securin and cyclin B1 cytoplasmic abundance to promote timely anaphase during meiosis in C. elegans Development 140 2149-2159 2013 Caenorhabditis elegans https://pubmed.ncbi.nlm.nih.gov/23578927/ 2.3.2.23 725950 High-yield expression in Escherichia coli and purification ofmouse ubiquitin-activating enzyme E1 Mol. Biotechnol. 51 254-261 2012 Mus musculus https://pubmed.ncbi.nlm.nih.gov/22012022/ 2.3.2.23 734317 Human liver cytochrome P450 3A4 ubiquitination: molecular recognition by UBC7-gp78 autocrine motility factor receptor and UbcH5a-CHIP-Hsc70-Hsp40 E2-E3 ubiquitin ligase complexes J. Biol. Chem. 290 3308-3332 2015 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/25451919/ 2.3.2.23 778454 Identification and characterization of physiological pairing of E2 ubiquitin-conjugating enzymes and E3 ubiquitin ligases Methods Mol. Biol. 2581 13-29 2023 Embryophyta https://pubmed.ncbi.nlm.nih.gov/36413307/ 2.3.2.23 779222 Identification of small-molecule binding sites of a ubiquitin-conjugating enzyme-UBE2T through fragment-based screening Protein Sci. 33 e4904 2024 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/38358126/ 2.3.2.23 716288 Identification of testis-specific ubiquitin-conjugating enzyme in the ascidian Ciona intestinalis Mol. Reprod. Dev. 77 640-647 2010 Ciona intestinalis https://pubmed.ncbi.nlm.nih.gov/20578064/ 2.3.2.23 776267 Identification of ubiquitin variants that inhibit the E2 ubiquitin conjugating enzyme, Ube2k ACS Chem. Biol. 16 1745-1756 2021 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/34397214/ 2.3.2.23 728491 In planta evidence for the involvement of a ubiquitin conjugating enzyme (UBI E2 Clade) in negative regulation of disease resistance Plant Mol. Biol. Rep. 31 323-334 2013 Nicotiana benthamiana - 2.3.2.23 728491 In planta evidence for the involvement of a ubiquitin conjugating enzyme (UBI E2 Clade) in negative regulation of disease resistance Plant Mol. Biol. Rep. 31 323-334 2013 Arabidopsis thaliana - 2.3.2.23 758642 Insights into the allosteric inhibition of the SUMO E2 enzyme Ubc9 Angew. Chem. Int. Ed. Engl. 55 5703-5707 2016 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/27038327/ 2.3.2.23 691518 Interactions between the quality control ubiquitin ligase CHIP and ubiquitin conjugating enzymes BMC Struct. Biol. 8 26 2008 Danio rerio https://pubmed.ncbi.nlm.nih.gov/18485199/ 2.3.2.23 733834 Investigations into the auto-FAT10ylation of the bispecific E2 conjugating enzyme UBA6-specific E2 enzyme 1 FEBS J. 281 1848-1859 2014 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/24528925/ 2.3.2.23 760147 Involvement of ubiquitin-conjugating enzyme (E2 gene family) in ripening process and response to cold and heat stress of Vitis vinifera Sci. Rep. 7 13290 2017 Vitis vinifera https://pubmed.ncbi.nlm.nih.gov/29038452/ 2.3.2.23 758749 Mechanism and disease association of E2-conjugating enzymes lessons from UBE2T and UBE2L3 Biochem. J. 473 3401-3419 2016 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/27729585/ 2.3.2.23 759768 Mechanism of lysine 48 selectivity during polyubiquitin chain formation by the Ube2R1/2 ubiquitin-conjugating enzyme Mol. Cell. Biol. 36 1720-1732 2016 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/27044868/ 2.3.2.23 759535 Multiple E2 ubiquitin-conjugating enzymes regulate human cytomegalovirus US2-mediated immunoreceptor downregulation J. Cell Sci. 130 2883-2892 2017 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/28743740/ 2.3.2.23 728610 Overexpression of VrUBC1, a mung bean E2 Ubiquitin-conjugating enzyme, enhances osmotic stress tolerance in Arabidopsis PLoS One 8 e66056 2013 Vigna radiata https://pubmed.ncbi.nlm.nih.gov/23824688/ 2.3.2.23 776887 Plant ubiquitin E2 enzymes UBC32, UBC33, and UBC34 are involved in ERAD and function in host stress tolerance BMC Plant Biol. 25 412 2025 Solanum lycopersicum https://pubmed.ncbi.nlm.nih.gov/40169946/ 2.3.2.23 776887 Plant ubiquitin E2 enzymes UBC32, UBC33, and UBC34 are involved in ERAD and function in host stress tolerance BMC Plant Biol. 25 412 2025 Arabidopsis thaliana https://pubmed.ncbi.nlm.nih.gov/40169946/ 2.3.2.23 674561 Pleiotropic effects of ATP*Mg2+ binding in the catalytic cycle of ubiquitin-activating enzyme J. Biol. Chem. 281 14729-14737 2006 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/16595681/ 2.3.2.23 759764 Posttranscriptional regulation of glycoprotein quality control in the endoplasmic reticulum is controlled by the E2 Ub-conjugating enzyme UBC6e Mol. Cell 63 753-767 2016 Mus musculus https://pubmed.ncbi.nlm.nih.gov/27570074/ 2.3.2.23 777321 Recruitment of ubiquitin E2 enzymes is determined jointly by the U-box domains and substrates of E3 ligases FEBS Lett. 598 702-715 2024 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/38439679/ 2.3.2.23 733322 Role of an N-terminal site of Ubc9 in SUMO-1, -2, and -3 binding and conjugation Biochemistry 42 9959-9969 2003 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/12924945/ 2.3.2.23 776519 Role of Ubiquitin-conjugating enzyme E2 (UBE2) in two immune-mediated inflammatory skin diseases a mendelian randomization analysis Arch. Dermatol. Res. 316 249 2024 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/38795139/ 2.3.2.23 778830 Role of ubiquitin-conjugating enzyme E2T in the carcinogenesis and progression of pancreatic cancer Oncol. Lett. 20 1462-1468 2020 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/32724389/ 2.3.2.23 735453 Serine/threonine kinases and E2-ubiquitin conjugating enzymes in Planctomycetes: unexpected findings Antonie van Leeuwenhoek 104 509-520 2013 Gemmata obscuriglobus https://pubmed.ncbi.nlm.nih.gov/23918348/ 2.3.2.23 735453 Serine/threonine kinases and E2-ubiquitin conjugating enzymes in Planctomycetes: unexpected findings Antonie van Leeuwenhoek 104 509-520 2013 Pirellula staleyi https://pubmed.ncbi.nlm.nih.gov/23918348/ 2.3.2.23 735453 Serine/threonine kinases and E2-ubiquitin conjugating enzymes in Planctomycetes: unexpected findings Antonie van Leeuwenhoek 104 509-520 2013 Pirellula staleyi DSM 6068 https://pubmed.ncbi.nlm.nih.gov/23918348/ 2.3.2.23 736063 Spectrin's chimeric E2/E3 enzymatic activity Exp. Biol. Med. 240 1039-1049 2015 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/26283706/ 2.3.2.23 728208 Steady-state kinetic analysis of human ubiquitin-activating enzyme (E1) using a fluorescently labeled ubiquitin substrate J. Protein Chem. 19 489-498 2000 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/11195973/ 2.3.2.23 777312 Structural and biophysical characterisation of ubiquitin variants that inhibit the ubiquitin conjugating enzyme Ube2d2 FEBS J. 291 5305-5321 2024 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/39473070/ 2.3.2.23 759820 Structural insights into E1 recognition and the ubiquitin-conjugating activity of the E2 enzyme Cdc34 Nat. Commun. 10 3296 2019 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/31341161/ 2.3.2.23 759820 Structural insights into E1 recognition and the ubiquitin-conjugating activity of the E2 enzyme Cdc34 Nat. Commun. 10 3296 2019 Saccharomyces cerevisiae https://pubmed.ncbi.nlm.nih.gov/31341161/ 2.3.2.23 759820 Structural insights into E1 recognition and the ubiquitin-conjugating activity of the E2 enzyme Cdc34 Nat. Commun. 10 3296 2019 Saccharomyces cerevisiae ATCC 204508 https://pubmed.ncbi.nlm.nih.gov/31341161/ 2.3.2.23 778804 Structure and functional determinants of Rad6-Bre1 subunits in the histone H2B ubiquitin-conjugating complex Nucleic Acids Res. 51 2117-2136 2023 Saccharomyces cerevisiae https://pubmed.ncbi.nlm.nih.gov/36715322/ 2.3.2.23 759478 Structure of UBE2Z enzyme provides functional insight into specificity in the FAT10 protein conjugation machinery J. Biol. Chem. 291 630-639 2016 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/26555268/ 2.3.2.23 733985 Systematic yeast two-hybrid analysis of human E2 ubiquitin-conjugating enzyme and deubiquitin (DUB) protein interactions Int. J. Biol. Chem. 7 1-14 2013 Homo sapiens - 2.3.2.23 778533 Targeting E2 ubiquitin-conjugating enzyme UbcH5c by small molecule inhibitor suppresses pancreatic cancer growth and metastasis Mol. Cancer 21 70 2022 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/35272681/ 2.3.2.23 734290 The E2 ubiquitin-conjugating enzyme UBE2J1 is required for spermiogenesis in mice J. Biol. Chem. 289 34490-34502 2014 Mus musculus https://pubmed.ncbi.nlm.nih.gov/25320092/ 2.3.2.23 725413 The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines J. Biol. Chem. 285 8595-8604 2010 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/20061386/ 2.3.2.23 778139 The E2 ubiquitin-conjugating enzymes UBE2D1 and UBE2D2 regulate VEGFR2 dynamics and endothelial function J. Cell Sci. 136 jcs260657 2023 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/37226882/ 2.3.2.23 723874 The expression, purification and crystallization of a ubiquitin-conjugating enzyme E2 from Agrocybe aegerita underscore the impactof His-tag location on recombinant protein properties Acta Crystallogr. Sect. F 69 153-157 2013 Cyclocybe aegerita https://pubmed.ncbi.nlm.nih.gov/23385757/ 2.3.2.23 724951 The family of ubiquitin-conjugating enzymes (E2s):deciding between life and death of proteins FASEB J. 24 981-93 2010 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/19940261/ 2.3.2.23 734161 The HECT domain of TRIP12 ubiquitinates substrates of the ubiquitin fusion degradation pathway J. Biol. Chem. 284 1540-1549 2009 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/19028681/ 2.3.2.23 759124 The ubiquitin E2 enzyme UBE2QL1 mediates lysophagy EMBO Rep. 20 e49104 2019 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/31523916/ 2.3.2.23 1111 The ubiquitin-activating enzyme (E1) gene family in Arabidopsis thaliana Plant J. 11 213-226 1997 Arabidopsis thaliana https://pubmed.ncbi.nlm.nih.gov/9076989/ 2.3.2.23 725526 The ubiquitin-conjugating enzyme (E2) Ube2w ubiquitinates the N terminus of substrates J. Biol. Chem. 288 18784-18788 2013 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/23696636/ 2.3.2.23 715517 The ubiquitin-conjugating enzyme UbcM2 can regulate the stability and activity of the antioxidant transcription factor Nrf2 J. Biol. Chem. 285 23064-23074 2010 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/20484052/ 2.3.2.23 779082 The ubiquitin-conjugating enzyme UBE2D maintains a youthful proteome and ensures protein quality control during aging by sustaining proteasome activity PLoS Biol. 23 e3002998 2025 Drosophila melanogaster https://pubmed.ncbi.nlm.nih.gov/39879147/ 2.3.2.23 733350 The ubiquitin-conjugating enzyme, UbcM2, is restricted to monoubiquitylation by a two-fold mechanism that involves backside residues of E2 and Lys48 of ubiquitin Biochemistry 53 4004-4014 2014 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/24901938/ 2.3.2.23 778698 Trim-Away ubiquitinates and degrades lysine-less and N-terminally acetylated substrates Nat. Commun. 14 2160 2023 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/37061529/ 2.3.2.23 734918 UBC13, an E2 enzyme for Lys63-linked ubiquitination, functions in root development by affecting auxin signaling and Aux/IAA protein stability Plant J. 80 424-436 2014 Arabidopsis thaliana https://pubmed.ncbi.nlm.nih.gov/25142088/ 2.3.2.23 776614 UbcH5 interacts with substrates to participate in lysine selection with the E3 ubiquitin ligase CHIP Biochemistry 59 2078-2088 2020 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/32401531/ 2.3.2.23 674842 UBE1L2, a novel E1 enzyme specific for ubiquitin J. Biol. Chem. 282 23010-23014 2007 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/17580310/ 2.3.2.23 778828 UBE2J1 is the E2 ubiquitin-conjugating enzyme regulating androgen receptor degradation and antiandrogen resistance Oncogene 43 265-280 2024 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/38030789/ 2.3.2.23 776946 Ubiquitin conjugating enzyme E2°C (UBE2C) may play a dual role involved in the progression of thyroid carcinoma Cell Death Discov. 8 130 2022 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/35332135/ 2.3.2.23 758765 Ubiquitin-conjugating enzyme E2 B regulates the ubiquitination of O6-methylguanine-DNA methyltransferase and BCNU sensitivity in human nasopharyngeal carcinoma cells Biochem. Pharmacol. 158 327-338 2018 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/30449727/ 2.3.2.23 759492 Ubiquitin-conjugating enzyme E2 D1 (Ube2D1) mediates lysine-independent ubiquitination of the E3 ubiquitin ligase March-I J. Biol. Chem. 293 3904-3912 2018 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/29414787/ 2.3.2.23 777687 Ubiquitin-conjugating enzyme E2S decreases the sensitivity of glioblastoma cells to temozolomide by upregulating PGAM1 via the interaction with OTUB2 Int. J. Biol. Macromol. 302 140583 2025 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/39904430/ 2.3.2.23 760224 Ubiquitin-conjugating enzyme E2T is an independent prognostic factor and promotes gastric cancer progression Tumour Biol. 37 11723-11732 2016 Homo sapiens https://pubmed.ncbi.nlm.nih.gov/27020591/