2.6.1.85 chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate association of PabA and PabB is greatly enhanced by 5 mM glutamine and greatly reduced at cold temperatures 644248 2.6.1.85 chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate in the absence of PabA and glutamine, PabB converts ammonia and chorismate into 4-amino-4-deoxychorismate (in the presence of Mg2+) 644230 2.6.1.85 chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate mechanism includes nucleophile addition to C2 of chorismate in an SN2'' process 661933 2.6.1.85 chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate non linear production of PABA in the ADC synthase-ADC lyase-coupled reaction system, at different glutamine and chorismate concentrations, with limiting PabB 644230 2.6.1.85 chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate omega-amino group of K274 adds to chorismate at C2, leading to elimination of the C4 hydroxyl in an SN2'' displacement reaction. After attack of ammonia to intermediate, another SN2'' displacement expels K274 and forms aminodeoxychorismate 661938 2.6.1.85 chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate overall reaction - 2.6.1.85 chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate the enzyme is composed of two parts, PabA and PabB 644230, 644248 2.6.1.85 chorismate + NH3 = 4-amino-4-deoxychorismate + H2O (1b) - 2.6.1.85 L-glutamine + H2O = L-glutamate + NH3 (1a) -