1.1.1.270 a 3beta-hydroxysteroid + NADP+ = a 3-oxosteroid + NADPH + H+ also acts on 5alpha-cholest-7-en-3-one - 1.1.1.270 a 3beta-hydroxysteroid + NADP+ = a 3-oxosteroid + NADPH + H+ residues Leu/Val54, Tyr55, His117, Val128, Ile129, His222, and Trp227 are involved in substrate binding, Tyr55, Asp50, Lys84, and His117 form the catalytic tetrad, Tyr55 and His117 together with NADP+ form an oxyanion hole through hydrogen bond networks, molecular docking simulations, detailed overview 677144 1.1.1.270 a 3beta-hydroxysteroid + NADP+ = a 3-oxosteroid + NADPH + H+ stereoselectivity : #1,4,5# catalyzes reduction of the 3-keto group to a 3beta-hydroxyl group <5,6,7> 286474, 286477