6.1.1.26 - 6.1.1.26 DhaPylSc 6.1.1.26 nickel-activated HiTrap column chromatography 6.1.1.26 purified by Ni-NTA chromatography and SDS gel electrophoresis 6.1.1.26 recombinant His-tagged enzyme from Escherichia coli BL21(DE3) 6.1.1.26 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 6.1.1.26 recombinant His-tagged N-terminally truncated enzyme form PylRS(c270) from Escherichia coli strain BL21(DE3) to homogeneity by affinity chromatography, hydrophobic interaction chromatography, and adsorption chromatography 6.1.1.26 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli BL21(DE3) 6.1.1.26 recombinant His6-tagged PylS from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 6.1.1.26 recombinant N-terminally His-tagged catalytic domain of the enzyme from Escherichia coli by two different steps of affinity chromatography, and gel filtration