5.3.3.2 - 5.3.3.2 2 isoforms: I and II 5.3.3.2 by affinity chromatography on Ni-NTA resin 5.3.3.2 Co2+ column chromatography, and gel filtration 5.3.3.2 cobalt column chromatography 5.3.3.2 DEAE Sepharose column chromatography and Resource Phe column chromatograpyh 5.3.3.2 His-Trap column chromatography 5.3.3.2 Ni-NTA agarose column chromatography 5.3.3.2 Ni-NTA agarose resin column chromatography 5.3.3.2 Ni-NTA column chromatography