5.1.1.15 - 5.1.1.15 ACL racemase is purified from Escherichia coli JM109/pACL60 5.1.1.15 native enzyme by several steps of ion exchange and hydrophobic interaction chromatography, dialysis, ultrafiltration, and gel filtration, recombinant His-tagged enzyme 31.9fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity 5.1.1.15 partial 5.1.1.15 recombinant 5.1.1.15 recombinant His-tagged enzyme 129fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis 5.1.1.15 recombinant His-tagged enzyme 27.9fold from Escherichia coli strain BL21(DE3) by heat treatment, nickel affinity chromatography, and dialysis 5.1.1.15 recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and dialysis 5.1.1.15 using Ni Sepharose highperformance (Amersham) and anion-exchange chromatography (HiTrapQFF)