4.2.1.36 - 5675 4.2.1.36 HisTrap HP column chromatography and Superdex 200 gel filtration 726596 4.2.1.36 recombinant wild-type and mutant small and large subunit proteins MJ1271 and MJ1003 from Escherichia coli strain BL21(DE3) by heat treatment at 70°C for 10 min, anion and cation exchange chromatography, adsorption chromatography, and gel filtration 702368