3.2.1.163 Cibacron blue F3GA resin column chromatography, Sepharose column chromatography, and Superdex 200 gel filtration 706628 3.2.1.163 native enzyme 370fold by anion-exchange chromatography, chromatofocussing, and gel filtration 664527 3.2.1.163 native enzyme partially by affinity chromatography 673892 3.2.1.163 recombinant His- or HA-tagged enzyme 674494