3.1.1.58	-	
3.1.1.58	140fold, AHU 7165	
3.1.1.58	homogeneity	
3.1.1.58	recombinant enzyme from Escherichia coli strain DE3 pLysS by cation exchange chromatography and gel filtration	
3.1.1.58	recombinant expression of wild-type and mutant enzymes from Escherichia coli	
3.1.1.58	recombinant N-terminally His10-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage through Factor Xa protease, and ulrafiltration/dialysis