2.7.4.2 - 2.7.4.2 ammonium sulfate, DEAE-cellulose, BioGel P-150, hydroxylapatite, Blue Dextran-Sepharose 2.7.4.2 ammonium sulfate, ethanol, phosphate gel, DEAE-cellulose 2.7.4.2 native enzyme from liver 2.7.4.2 Ni-NTA resin column chromatography 2.7.4.2 Ni-Sepharose column chromatography 2.7.4.2 Q Sepharose, Phenyl Sepharose, Sephacryl S200, Mono Q, gel filtration, Shodex KW803 2.7.4.2 recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography 2.7.4.2 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography 2.7.4.2 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration