2.4.1.9 - 2.4.1.9 AF-Chelate-650M column chromatography 2.4.1.9 Ni-NTA column chromatography 2.4.1.9 Ni2+-chelating Sepharose column chromatography 2.4.1.9 partial 2.4.1.9 partially purified by Toyopearl DEAE-650M column chromatography 2.4.1.9 recombinant enzyme 2.4.1.9 recombinant His-tagged full-length wild-type enzyme and truncated mutant enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and ultrafiltration 2.4.1.9 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli TOP10 cells by nickel affinity chromatography 2.4.1.9 recombinant His6-tagged truncated enzyme mutant from Escherichia coli strain DH10B by nickel affinity chromatography