2.3.3.8 - 2.3.3.8 ammonium sulfate precipitation, ion exchange chromatography (DEAE), gel filtration 2.3.3.8 brain enzyme 2.3.3.8 glutathione Sepharose bead chromatography 2.3.3.8 immobilized metal-ion affinity chromatography 2.3.3.8 liver enzyme 2.3.3.8 mammary gland 2.3.3.8 Ni-NTA agarose column chromatography, Q-Sepharose column chromatography and DEAE-Sephacel gel filtration 2.3.3.8 Ni-NTA column chromatography 2.3.3.8 Ni-NTA column chromatography, and gel filtration