2.3.1.133	-	
2.3.1.133	crude extracts from stem material from various down-regulated and control lines are assayed for extractable HCT enzyme activity	
2.3.1.133	partial	
2.3.1.133	recombinant enzyme	
2.3.1.133	recombinant GST-tagged HCT from Escherichia coli strain BL21 (DE3) by glutathione affinity chromatography	
2.3.1.133	recombinant GST-tagged PeHCT-1, PeHCT-2, PeHCT-3, PeHCT-4, PeHCT-5, PeHCT-6, and PeHCT-7 gene products from Escherichia coli strain BL21 (DE3) by glutathione affinity cromatography	
2.3.1.133	recombinant His-tagged HST from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and dialysis	
2.3.1.133	recombinant N-terminally His6-tagged HCT from Escherichia coli strain BL21 Star(DE3)pLysS by nickel affinity chromatography, dialysis, and cleavage of the tag by TEV protease, followed by gel filtration	
2.3.1.133	soluble recombinant GST-tagged OsHCT4 from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography	
2.3.1.133	tracheary elements are cultured