2.1.1.34 - 637158 2.1.1.34 affinity chromatography on S-adenosyl-L-homocysteine-Sepharose, elution with 6 M urea 637157 2.1.1.34 gel filtration, more than 99% pure 671082 2.1.1.34 native and recombinant from Escherichia coli 637164 2.1.1.34 wild-type and mutants purified by gel filtration, mutants R11A and R166A purified by repeated ion-exchange chromatography, to homogeneity 674692