1.4.3.16 - 391756, 391757, 391758, 391760, 721394, 742229, 743783, 764137, 764649, 765612 1.4.3.16 ammonium sulfate precipitation and Butyl Toyopearl column chromatography 685396 1.4.3.16 HiTrap nickel chelating affinity column chromatography, and Superdex 200 gel filtration 721394 1.4.3.16 HiTrap nickel chelating column chromatography and Superdex 75 gel filtration 725741 1.4.3.16 overexpression in Escherichia coli 391761, 391762, 391764, 391765 1.4.3.16 using NH4SO4 precipitation and using a DEAE-sepharose column 686712