1.3.3.11	-	
1.3.3.11	partial purification of recombinant protein	
1.3.3.11	Q-Sepharose column chromatography and Ultrogel AcA54 gel filtration	
1.3.3.11	recombinant His-tagged wild-type and mutant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration	
1.3.3.11	Superdex S-200 gel filtration