1.3.1.85 - 713409 1.3.1.85 DEAE-Sepharose column chromatography and Cibacron Blue 3GA agarose column chromatography 689745 1.3.1.85 HisTrap column chromatography 760072 1.3.1.85 Ni-NTA column chromatography and Superdex 200HR gel filtration 739814