1.2.7.1 - 1.2.7.1 DE52 column chromatography, DEAE-cellulose column chromatography, and Sephadex G25 gel filtration 1.2.7.1 enzyme Ape2126/2128 1.2.7.1 gel filtration 1.2.7.1 Ni-NTA column chromatography 1.2.7.1 nickel affinity column chromatography 1.2.7.1 purifiaction using Q-sepharose, hydroxyapatite, blue sepharose and sephacryl 1.2.7.1 purification from cells grown on glucose 1.2.7.1 purification under strictly unaerobic conditions 1.2.7.1 purified enzyme shos a brownish colour characteristic of iron-sulfur containing proteins