1.14.11.17	-	
1.14.11.17	DEAE-Sepharose column chromatography and HP phenyl-Sepharose column chromatography	
1.14.11.17	dialysis against 25 mM Tris buffer at pH 8.0	
1.14.11.17	recombinant His-tagged TauD from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	
1.14.11.17	recombinant protein using His-tag	
1.14.11.17	Resource-Q anion-exchange column chromatography and Superdex 200 gel filtration	
1.14.11.17	two-step purification from overexpressing Escherichia coli to apparent homogeneity