1.1.5.9	-	
1.1.5.9	by DEAE-5PW and Superdex-200 10/300GL column chromatography	
1.1.5.9	DEAE-5PW column chromatography and Superdex-200 gel filtration	
1.1.5.9	HisTrap nickel affinity chromatography	
1.1.5.9	isopropanol precipitation and nickel affinity column chromatography	
1.1.5.9	native extracellular enzyme 23.7fold from culture supernatant by anion exchange and hydrophobic interaction chromatography, ammonium sulfate fractionation, and ultrafiltration	
1.1.5.9	Ni-NTA agarose column chromatography	
1.1.5.9	recombinant enzyme from Escherichia coli strain BL21RIL	
1.1.5.9	recombinant enzyme from Pichia pastoris strain X-33 5.1fold by hydrophobic interaction and anion exchange chromatography	
1.1.5.9	recombinant enzyme from Pichia pastoris to homogeneity