1.1.1.363 - 1.1.1.363 a fast, efficient and reproducible procedure for the purification of the Pseudomonas aeruginosa enzyme that can be implemented in a short period (2 days). The method is based on protein precipitation with CaCl2 and further purification by ion exchange chromatography 1.1.1.363 mutant enzymes H250N, D177N and H178N