1.1.1.346 - 1.1.1.346 ammonium sulfate precipitation, DEAE Toyopearl column chromatography and phenyl Toyopearl column chromatography, Blue Sepharose column chromatography and Sephacryl S-200 gel filtration 1.1.1.346 ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Amicon Matrix Gel Red A gel filtration 1.1.1.346 ammonium sulfate precipitation, DEAE-Sepharose column chromatography, Matrex Red-A column chromatography, Sephacryl S-200 gel filtration, and hypatite C cholumn chromatography 1.1.1.346 DEAE cellulose resin column chromatography, gel filtration 1.1.1.346 DEAE-cellulose column chromatography, Cibacron blueF 3GA affinity column chromatography, and and TSK gel filtration 1.1.1.346 DEAE-Sephacel column chromatography and Cibacron blue 3GA column chromatography 1.1.1.346 Ni-NTA column chromatography 1.1.1.346 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography