1.1.1.263	gel filtration	
1.1.1.263	Q-Sepharose HP column chromatography, Superdex 200 HR10 30 column chromatography and Red Sepharose CL-6B chromatography	
1.1.1.263	recombinant enzyme 17.4fold from Escherichia coli strain BL21(DE3) by anion exchange and adsorption chromatography, and gel filtration	
1.1.1.263	to homogeneity, chromatography techniques