6.2.1.45	-	
6.2.1.45	His6-tagged E1 expressed and purified in Saccharomyces cerevisiae MHY-501 cells	
6.2.1.45	Ni-NTA column chromatography	
6.2.1.45	recombinant enzyme, purification on Ni-NTA superflow sepharose and strep-tactin sepharose which is based on UB-UBE1 high-energy thioester bonded intermediate complex	
6.2.1.45	recombinant FLAG-tagged wild-type and mutant enzmyes from Escherichia coli strain BL21 by affinity chromatography	
6.2.1.45	recombinant GST-tagged enzyme from Hi5 insect cells by glutathione affinity chromatography, tag cleavage by thrombin, and gel filtration	
6.2.1.45	recombinant His-tagged chimeric mutant Aos1-Uba2 SUMO-E1 enzyme mAU from insect cells by nickel affinity chromatography	
6.2.1.45	recombinant His-tagged enzyme domains FCCH, 4HB, SCCH and UFD from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, incase of domain UFD purification containing 6 M urea, followed by dialysis, and ultrafiltration	
6.2.1.45	recombinant His-tagged N-terminal enzyme domains from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, anion exchange chromatography, tag cleavage by 3C protease, another step of nickel affinity chromatography, followed by gel filtration	
6.2.1.45	recombinant His-tagged wild-type and mutant enzmyes from Escherichia coli strain BL21 by nickel affinity chromatography