5.4.2.7 - 3340, 3346, 715660 5.4.2.7 HisTrap column chromatography 748271 5.4.2.7 HisTrap HP column chromatography and Superdex 200 gel filtration 713648 5.4.2.7 IMAC Ni-charged resin column chromatography, and Superdex 200 gel filtration 746733 5.4.2.7 multiple forms: I, II, II-1 and III-2 3343 5.4.2.7 partial 662012 5.4.2.7 recombinant His-tagged Pgm1, Pgm2, and Pgm3 from Escherichia coli strain BL21 (DE3) 727521 5.4.2.7 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, His-tag removal by thrombin cleavage, followed by gel filtration 726977 5.4.2.7 recombinant PPM, Toyopearl HW-55, DEAE-Toyopearl, Butyl-Toyopearl, Sephacryl S-330 3348