4.3.2.2	-	1538, 1541, 34449, 34451, 34454, 34457, 665595, 749293	
4.3.2.2	Ni-affinity chromatography	664041	
4.3.2.2	Ni-NTA agarose column chromatography	707497	
4.3.2.2	normal and mutant enzyme S413P	34461	
4.3.2.2	Q-Sepharose column chromatography and gel filtration	706979	
4.3.2.2	recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta 2(DE3)pLysS by nickel affinity chromatography	729296	
4.3.2.2	recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta 2(DE3)pLysS by nickel affinity chromatography, dialysis, cleavage of the His-tag through thrombin, and gel filtration, to homogeneity	729218	
4.3.2.2	recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain Bl21 by nickel affinity chromatography	690934	
4.3.2.2	using a Ni-NTA column	681441	
4.3.2.2	using a Ni-NTA column, the 6-histidine tag is removed by cleavage with thrombin, separation of thrombin and histidine-tag from the enzyme is achieved by using a Sephadex G-100 column	682657