2.7.6.2 - 2.7.6.2 nickel-chelate chromatography 2.7.6.2 partial 2.7.6.2 recombinant CA1462 from Escherichia coli 2.7.6.2 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by cobalt affinity chromatography