2.1.1.224 C-terminally His6-tagged wild-type enzyme and mutant enzymes C112A, C116A and C119A 704203 2.1.1.224 recombinant His6-tagged Cfr from Escherichia coli strain BL21Star by nickel affinity chromatography and gel filtration 720968 2.1.1.224 recombinant tagged enzyme Cfr(E) from Escherichia coli strain Rosetta(DE3)pLysS by metal chelating chromatography 755810