1.3.7.3 - 437673 1.3.7.3 by gel filtration 674639 1.3.7.3 by two affinity chromatography steps to 90% purity and a third gel filtration step 674639 1.3.7.3 GST-tagged PebA forms inactive aggregates 742861 1.3.7.3 recombinant GST-tagged or His-tagged PebB from Escherichia coli BL21(DE3) by glutathione or nickel affinity chromatography, gel filtration, and ultrafiltration, recombinant tagged PebAgB by affinity chromatography 763020 1.3.7.3 recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli by DNA removal from cell extract, ultracentrifugation at 220000 x g, glutathione affinity chromatography and cleavage of the tag through TEV protease, followed by dialysis and a second step of glutathione affinity chromatography to remove that tag, gel filtration, and ultrafiltration 763264