1.13.11.3 - 439480, 439482, 439483, 439498, 439502, 439503, 439506, 439507, 665455, 666009, 685485, 763959 1.13.11.3 a highly active enzyme without nuclease contaminations is purified by Ni-NTA affinity chromatography and size exclusion chromatography for prolonged single-molecule fluorescence imaging 763879 1.13.11.3 ammonium sulfate, DEAE-cellulose 439488 1.13.11.3 ammonium sulfate, DEAE-cellulose, Bio-gel agarose, DEAE-cellulose 439501 1.13.11.3 ammonium sulfate, DEAE-cellulose, Sephadex G-200 439500 1.13.11.3 ammonium sulfate, DEAE-Sepharose, octyl-Sepharose, Phenyl-sepharose, recombinant enzyme 439508 1.13.11.3 ammonium sulfate, DEAE-Sepharose, Phenyl-Sepharose 439484 1.13.11.3 ammonium sulfate, Q-Sepharose, partial purification 439514 1.13.11.3 ammonium sulfate, Sephacryl S 300, DEAE-Sephadex A50 439499 1.13.11.3 cells harvested by centrifugation, washed twice with 50 mM sodium phosphate buffer, pH 7.0 containing 10% glycerol, disruption with solicitor, centrifuged, supernatant stored at -20°C 697244