7.2.2.14 - 7.2.2.14 native enzyme partially by mitochondria preparation 7.2.2.14 native enzyme partially from hearts by mitochondria preparation 7.2.2.14 Ni-NTA column chromatography 7.2.2.14 periplasmic domain of recombinant enzyme 7.2.2.14 recombinant enzyme, purification omits the isolation and subsequent solubilization of the membrane fraction 7.2.2.14 recombinant HA-tagged wild-type and mutant enzymes in yeast, native enzyme partially by preparation of mitochondria 7.2.2.14 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 by nickel affinity chromatography, cleavage of the His-tag, and gel filtration to homogeneity 7.2.2.14 recombinant N-terminally His-tagged cytosolic domain by nickel affinity chromatography, tag cleavage by thrombin, anion exchange chromatography and gel filtration, recombinant N-terminally His-tagged full-length MgtE from Escherichia coli strain C41 (DE3) by nickel affinity chromatography and gel filtration 7.2.2.14 recombinant periplasmic domain, glutathione S-transferase fusion protein